Background: T helper 17 (Th17) cells and the related cytokines, interleukin (IL)- 17 and IL-23, were proved to play pivotal roles during the development of allergic rhinitis (AR). IL-27, an anti-inflammatory cytokine, has been reported to promote the production of IL-12R and induce Th1 cell responses. However, its effect on Th17 responses was not fully understood. Objective: We conducted the present research to explore the role of IL-27 in the regulation of Th17 responses in AR. Methods: Thirty confirmed AR patients and 20 controls were recruited for the study. The mRNA expression and protein levels of IL-27 were analyzed employing quantitative PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA), respectively, and their correlations with Th17 cytokines were analyzed. We utilized ELISA and qPCR to analyze the effect of IL-27 on the differentiation of Th17 cells and the production of IL-17 and IL-23 from peripheral blood mononuclear cells (PBMCs). Results: We found that the IL-27 levels in AR were downregulated and negatively related to IL-17 and IL-23 levels. The recombinant IL-27 inhibited the mRNA expression of RORγt and the protein expression of IL-17 and IL-23 in PBMCs through MEK, NF-κB, and JNK pathways. Conclusion: Our data demonstrated that IL-27 suppressed Th17 responses through MEK, NF-κB, and JNK pathways.
Ouyang, H., Cheng, J., Du, J., Gan, H. & Lu, Z. (2020). Interleukin-27 Suppresses T Helper-17 Inflammation in Allergic Rhinitis. Iranian Journal of Immunology, 17(4), 275–282. https://doi.org/10.22034/iji.2020.84871.1675
MLA
Ouyang, H., Cheng, J., Du, J., Gan, H., & Lu, Z. "Interleukin-27 Suppresses T Helper-17 Inflammation in Allergic Rhinitis", Iranian Journal of Immunology, 17, 4, 2020, 275–282. doi: 10.22034/iji.2020.84871.1675
HARVARD
Ouyang, H., Cheng, J., Du, J., Gan, H., & Lu, Z. (2020). 'Interleukin-27 Suppresses T Helper-17 Inflammation in Allergic Rhinitis', Iranian Journal of Immunology, 17(4), pp. 275–282. doi: 10.22034/iji.2020.84871.1675
CHICAGO
Ouyang, H., Cheng, J., Du, J., Gan, H. & Lu, Z., "Interleukin-27 Suppresses T Helper-17 Inflammation in Allergic Rhinitis." Iranian Journal of Immunology, 17 4 (2020): 275–282, doi: 10.22034/iji.2020.84871.1675
VANCOUVER
Ouyang H., Cheng J., Du J., Gan H., Lu Z. Interleukin-27 Suppresses T Helper-17 Inflammation in Allergic Rhinitis. Iran.J.Immunol. 2020;17(4):275–282. doi: 10.22034/iji.2020.84871.1675
