Document Type : Original Article

Authors

• Ahana Roy ¹
• Aitijhya Ghosh ¹
• Kasturi Sinha ¹
• Basundhara Mitra ¹
• Sudeshna Rakshit ¹
• Melvin George ²
• Koustav Sarkar ¹

¹ SRM Research Institute and Department of Biotechnology, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203, India

² Department of Clinical Pharmacology, SRM Medical College Hospital and Research Center, Kattankulathur, Tamil Nadu 603203, India

Abstract

Background: Breast cancer is an uncontrolled growth of epithelial cells. The loss of BRCA1 activity due to mutation or down-regulation of gene expression promotes tumorigenesis and increases the risk of breast cancer.
Objectives: Our aim was to pulsate lymphocytes of breast cancer patients and normal individuals, using Diospyros peregrina fruit preparation (DFP) to study the cancer protective immunity, and the signal transduction processes involved with it. We also investigated the role of DFP in the release of lymphocytic nitric oxide (NO), which is a key tumoricidal agent, known to regulate T-cell proliferation, cytokine production, cell signaling, and apoptosis.
Methods: Using Ficoll-Hypaque gradient centrifugation, lymphocytes were isolated from the blood of 12 patients and 12 normal individuals. Cells were treated with or without DFP (2.5 µg/ml) for 48 hours. Both non-stimulated and stimulated cells were then subjected to MTT assay and NO release assay; following which qPCR was performed to estimate mRNA levels and percentage enrichment of certain genes.
Results: DFP stimulates lymphocytic proliferation(p=0.0118) and release of NO(p=0.01) significantly.DFP also noticeablyenhances the expression of T helper (TH) cell 1 specific IFNG, IL12, TBX21 and signal transducer and activator of transcription 1 (STAT1) genes. DFP treatment significantly increases tumor protective immunity by decreasing the expression levels of TH2 network specific GATA3 and IL4 genes but increasing the expression levels of TH1 network specific IFNG, IL12, TBX21 and STAT1 genes.
Conclusion: DFP increases the expression levels TH1 specific network genes which in turn help in evoking tumor protective immunity.

Keywords

• Diospyros peregrina fruit preparation (DFP)
• Nitric Oxide (NO)
• T helper (TH) cell
• Breast Cancer