Iranian Journal of Immunology

Teucrium polium Extract Attenuates Inflammation in Asthma by Reducing RORγt Transcription and Increasing IL-10 Secretion in an Ovalbumin-induced Murine Asthma Model

Document Type : Original Article

Authors

Shole Daneshvar-ghahfarokhi 1
Amir Rahnama 2
Vahid Mohammadi-Shahrokhi 1, 3

1 Department of Immunology, Faculty of Medicine, Rafsanjan University of Medical, Rafsanjan, Iran.

2 Department of Pathology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

3 Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

https://doi.org/10.22034/iji.2023.97422.2509
Abstract
Background: One of the inflammatory diseases of the respiratory system is asthma. Teucrium polium (TP) has anti-inflammatory and anti-allergic properties and its anti-asthmatic effects have not been investigated yet. RORγt is an inflammatory transcription factor for Th17 differentiation. By secreting IL-17, Th17 leads to neutrophilic inflammation in the lungs. As an anti-inflammatory cytokine, IL-10 reduces the dissemination of inflammatory elements in the airways.
Objective: To evaluate the effect of TP extract in asthma treatment.
Methods: Thirty female Balb/c mice were distributed into 5 groups (n=6) including the control, treated with ovalbumin (OVA), and OVA+ various doses of TP (50, 150, and 300 mg/kg). All groups except the control group were sensitized to OVA solution on days 0, 7, and 14 by subcutaneous injection. The challenge was performed on days 18 to 21 by the inhalation of 1% OVA and the treatment was done with TP extract in the treatment groups, half an hour before the challenge. On day 22, the serum and spleen samples were collected to determine IL-10 serum levels and RORγt gene expression, respectively.
Results: In the treatment groups, the expression of RORγt significantly decreased when using OVA+ Tp extract (150 mg/kg and 300 mg/kg), and IL-10 serum levels significantly increased when using OVA+ TP extract (150 mg/kg) compared with the OVA group.
Conclusion: It is possible that TP extract can be effective in improving asthma by reducing inflammation.

Keywords

Asthma
Extract
IL-10
Inflammation
RORγt
Teucrium polium
  1. Castillo J, Peters S, Busse W. Asthma exacerbations: pathogenesis, prevention, and treatment. The journal of allergy and clinical immunology in practice. 2017;5(4):918-27.
  2. Dharmage SC, Perret JL, Custovic A. Epidemiology of asthma in children and adults. Front pediatr. 2019;7:246.
  3. Choy Df, Hart K, Borthwick L, Shikotra A, Nagarkar D, Siddiqui S. TH2 and TH17 inflammatory pathways are reciprocally regulated in asthma. Science translational medicine. 2015;7(301):301-29.
  4. Jin D, Zhang L, Zheng J, Zhao Y. The inflammatory Th17 subset in immunity against self and non-self antigens. Autoimmunity. 2008;41(2):154-62.
  5. Ramakrishnan R, Al Heialy S, Hamid Q. Role of IL-17 in asthma pathogenesis and its implications for the clinic. Expert review of respiratory medicine. 2019;13(11):1057-68.
  6. Newcomb D, Peebles R, Jr. Th17-mediated inflammation in asthma. Curr opin immunol. 2013;25(6):755-60.
  7. Jovanovic D, Dibattista J, Martelpelletier J, Jolicoeur F, He Y, Zhang M. IL-17 stimulates the production and expression of proinflammatory cytokines, IL-1beta and TNF-alpha, by human macrophages. J Immunol. 1998;160(7):3513-21.
  8. Fiorentino D, Zlotnik A, Mosmann T, Howard M, O'Garra A. IL-10 inhibits cytokine production by activated macrophages. J Immunol. 1991;147(11):3815-22.
  9. Boonpiyathad T, Satitsuksanoa P, Akdis M, Akdis C. Il-10 producing T and B cells in allergy. Seminars in immunology. 2019;44:101326.
  10. John M, Lim S, Seybold J, Jose P, Robichaud A, O'Connor B. Inhaled corticosteroids increase interleukin-10 but reduce macrophage inflammatory protein-1alpha, granulocyte-macrophage colony-stimulating factor, and interferon-gamma release from alveolar macrophages in asthma. American journal of respiratory and critical care medicine. 1998;157(1):256-62.
  11. Ogawa Y, Duru E, Ameredes B. Role of IL-10 in the resolution of airway inflammation. Current molecular medicine. 2008;8(5):437-45.
  12. Castro MS, Azpiroz M, Molina M,Mourelle A, Alaniz F, Maldonado A. Preliminary studies on the prevention of the ovalbumin-induced allergic response by Enterococcus faecalis CECT7121 in mice. International archives of allergy and immunology. 2012;157(1):11-20.
  13. Boulila A, Bejaoui A, Messaoud C, Boussaid M. Genetic diversity and population and structure of Teucrium polium (Lamiaceae) in Tunisia. 2009;48:57-70.
  14. Sheikhbahaei F, Nematollahi-Mahani S, Khazaei M, Khazaei M, Khazayel S. Teucrium polium Extract enhances the anti-angiogenic effect of tranilast in a three-dimensional fibrin matrix model. Asian pac j cancer prev. 2021;22(8):2471-8.
  15. Rezaei Z, Nazerizadeh M, Seadat-Mahani M, Esmaeili Mahani S. Oral administration of Teucrium polium extract ameliorates ovalbumin-induced allergy in rats. The Journal of Physiology and pharmacology. 2013;17.
  16. Nassar A, Al-Mukhtar N, Alturaihy S. Physiological effects of Teucrium polium extract ameliorates ovalbumin-induced allergy in rabbits. 2009.
  17. Abdollahi M, Karimpour H, Monsef-Esfehani H. Antinociceptive effects of Teucrium polium L. total extract and essential oil in mouse writhing test. 2003;48(1):31-5.
  18. Heidari M, Karaminejhad ranjbar M, Dadvand E, Jalali S. Evaluation of the analgesic effect of Teucrium polium extract in mice. 1999;6(2):67-76.
  19. Chauhan P, Singh R. Ovalbumin-induced allergic inflammation lead to structural alterations in mouse model and protective effects of intranasal curcumin: A comparative study. Allergologia et immunopathologia. 2016;44(3):246-56.
  20. Kim S, Lee D, Jung W, Kim J, Choi I, Park S. Effects of Ecklonia cava ethanolic extracts on airway hyperresponsiveness and inflammation in a murine asthma model: Role of suppressor of cytokine signaling. Biomedicine & pharmacotherapy. 2008;62(5):289-96.
  21. Kianmehr M, Haghmorad D, Nosratabadi R, Rezaei A, Alavinezhad A, Boskabady M. The Effect of Zataria multiflora on Th1/Th2 and Th17/T Regulatory in a Mouse Model of allergic asthma. Front pharmacol. 2017;8:458.
  22. Mohammadi shahrokhi V, Rezaei A, Andalib A, Rahnama A, Jafarzadeh A, Eskandari N. Improvement of Th1/Th2 and Th1/Treg imbalances by adjutants CPG, MPLA and BCG in a model of acute asthma induced by allergen Derp2 in BALB/c Mice. Iranian red crescent medical journal. 2016.
  23. Mohammadishahrokhi V, Rezaei A, Andalib A, Rahnama A, Jafarzadeh A, Eskandari N. Improvement of TH1/TH2 and TH1/TREG imbalances by adjutants CPG, MPLA and BCG in a model of acute asthma induced by allergen derp2 in Balb/c mice. Inflammation.2017;19(3).
  24. Murdoch J, Lloyd C. Chronic inflammation and asthma. Mutation Research/fundamental and molecular mechanisms of mutagenesis. 2010;690(1):24-39.
  25. Rahmouni F, Hamdaoui L, Rebai T. In vivo anti-inflammatory activity of aqueous extract of Teucrium polium against carrageenan-induced inflammation in experimental models. Archives of physiology and biochemistry. 2017;123(5):313-21.
  26. Santiago L, Neto R, Santos ataide A, Fonseca D, Soares E, De Sa sousa J. Flavonoids, alkaloids and saponins: are these plant-derived compounds an alternative to the treatment of rheumatoid arthritis? A literature review. Clinical phytoscience. 2021;7(1):58.
  27. Pathak M, Patowary P, Goyary D, Das A, Chattopadhyay P. β-caryophyllene ameliorated obesity-associated airway hyperresponsiveness through some non-conventional targets. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2021;89:153610.
  28. Raeiszadeh jahromi S, Mahesh P, Jayaraj B, Madhunapantula S, Holla A, Vishweswaraiah S. Serum levels of IL-10, IL-17F and IL-33 in patients with asthma: a case control study. Journal of asthma. 2014;51:1004 - 13.
  29. Nabe T, Ikedo A, Hosokawa F, Kishima M, Fujii M, Mizutani N. Regulatory role of antigen-induced interleukin-10, produced by CD4(+) T cells, in airway neutrophilia in a murine model for asthma. European journal of pharmacology 2012;677 1-3:154-62.
  30. Chang S. T helper 17 (Th17) cells and interleukin-17 (IL-17) in cancer. Archives of pharmacal research. 2019;42(7):549-59.
  31. Naufel A, Aguiar M, Madeira M, Guimaraes abreu L. Treg and Th17 cells in inflammatory periapical disease: a systematic review. Brazilian oral research. 2017;31.
  32. Louten J, Boniface K, De waal malefyt R. Development and function of TH17 cells in health and disease. Journal allergy clin immunol. 2009;123(5):1004-11.
  33. Wei Q, Liao J, Jiang M, Liu J, Liang X, Nong G. Relationship between Th17-mediated immunity and airway inflammation in childhood neutrophilic asthma. Allergy asthma clinical immunology 17, 4 (2021).
  34. Latifynia A, Gharagozlou M, Mohebali M, Hajjaran H, Khansari N. Th1, Th2 Serum cytokines and spleen white pulp changes against preliminary L. major vaccine injection and challenge with live L. major Promastigotes in Balb/C Mice. Journal of clinical & cellular immunology 1-9. 2015;6:1-9.
Iranian Journal of Immunology
Volume 20, Issue 2
Spring 2023
Pages 159-166

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