Zeinab Tavakkol Afshari; Hamid Reza Rahimi; Seyed Morteza Ehteshamfar; Rashin Ganjali; Fatemeh Tara; Abbas Shapouri Moghadam
Volume 13, Issue 4 , December 2016, , Pages 309-316
Abstract
Background: Pre-eclampsia is the most common critical condition during pregnancy.
Plasma concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-1-beta
(IL-1β) increase in pregnant women with pre-eclampsia, compared to normal pregnant
women. Objective: To investigate the polymorphisms ...
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Background: Pre-eclampsia is the most common critical condition during pregnancy.
Plasma concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-1-beta
(IL-1β) increase in pregnant women with pre-eclampsia, compared to normal pregnant
women. Objective: To investigate the polymorphisms of IL-1β (C+3954T), TNF-α (G-
308A), and (G-238A) in preeclemptic women northeastern Iran. Methods: This study
was conducted on 153 preeclamptic women (case group) and 150 healthy pregnant
women (control group), admitted to Ghaem and Imam Reza hospitals of Mashhad, Iran.
IL-1β (C+3954T), TNF- α (G-238A) and TNF-α (G-308A) gene polymorphisms in the
promoter region were screened by polymerase chain reaction. Data were analyzed,
using SPSS version 16.0. Results: The mean age of the participants in the case and
control groups was 28.2 ± 6.1 and 27.1 ± 6.3 years, respectively (P=0.68). The
frequency of G-308A polymorphism was significantly higher in the case group,
compared to the control group (p<0.001). However, no significant relationship was
found between IL-1β genotype and pre-eclampsia (p=0.39). The frequency of TNF- α
(G-238A) AA genotype was significantly higher in the case group, while GG genotype
was less frequently detected in the case group, compared to the control group (p<0.001
for both genotypes). Moreover, the frequencies of AA genotypes of -238 TNF-α and G-
308A polymorphisms were significantly higher in the case group, compared to the
control group (p<0.001). Conclusion: The significant correlation between inflammation
promoting genotypes of TNF-α and Pre-eclampsia is noteworthy and provides evidence
on the contribution of immune related genes in this disease.
Motahareh Bahadori; Saeed Zarei; Amir Hassan Zarnani; Omid Zarei; Farah Idali; Reza Hadavi; Mahmood Jeddi-Tehrani
Volume 11, Issue 2 , June 2014, , Pages 97-104
Abstract
Background: Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM). Objective: To investigate the association between RM and five polymorphisms of cytokine genes, interleukin 10 (IL-10), (-592 ...
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Background: Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM). Objective: To investigate the association between RM and five polymorphisms of cytokine genes, interleukin 10 (IL-10), (-592 A/C, -819 C/T, -1082 A/G), IL-6 (-174 C/G) and IL-17 (-197 G/A) in Iranian women. Method: Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) was performed to determine the frequencies of the IL-6, IL-10 and IL-17 gene polymorphisms in 85 women with RM compared with 104 healthy controls. Results: The frequencies of IL- 10 promoter gene polymorphisms (-592 A/C and -819 C/T) were significantly higher in RM women than those in controls (p=0.003). However, no statistically significant differences were observed in the frequencies of IL-6 (-174 C/G), IL-10 (-1082 A/G) and IL-17 (-197 G/A) polymorphisms between RM women and controls. Conclusion: These results suggest that IL-10 gene polymorphism screening might have some relevance in patients with RM, a suggestion which requires further studies.
Nader Tajik; Tohid Kazemi; Aliakbar Delbandi; Ahad Ghods; Alireza Salek Moghaddam
Volume 3, Issue 4 , December 2006, , Pages 150-156
Abstract
Background: In addition to Human Leukocyte Antigens (HLA) compatibility, gene polymorphisms in cytokines might also be important in the quality of allogeneic immune response. Objective: To evaluate the influence of HLA-DR matching and a number of cytokine gene polymorphisms on acute rejection after living-unrelated ...
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Background: In addition to Human Leukocyte Antigens (HLA) compatibility, gene polymorphisms in cytokines might also be important in the quality of allogeneic immune response. Objective: To evaluate the influence of HLA-DR matching and a number of cytokine gene polymorphisms on acute rejection after living-unrelated donor (LURD) kidney transplantation. Methods: A total of 42 renal transplants performed at Hashemi Nejad Kidney Hospital (Tehran/Iran) and followed up for 3 months post-transplantation were included. Using PCR-SSP, HLA-DR alleles (DR1- 18) of recipients and donors and gene polymorphisms in TNF-a, TGF-b1, IL-10, IL- 6, and IFN-g of recipients were determined. Results: Acute rejection was observed in 11(26.2%) of renal recipients. The frequency of one and two HLA-DR mismatches in rejector group was 2(18.2%) and 9(81.8%) and in non-rejector group was 13(41.9%) and 17(54.8%), respectively. HLA-DR incompatibility was not significantly higher in rejector (1.82 0.40) compared with non-rejector (1.52 0.57) recipients (P=0.069) and more than half of non-rejectors had completely mismatched HLA-DR antigens with donors. Polymorphisms associated with the mentioned cytokines had no correlation with acute rejection. Conclusion: The predictive value of HLA-DR mismatching for acute rejection is not as prominent in LURD kidney transplantation as in the cadaveric one. In addition, we failed to demonstrate an association between combined cytokine genotypes and HLA-DR matching with acute rejection. Further and more detailed immunogenetic investigations are required in order to have a better prediction of the transplant outcome.
