Document Type: Original Article

Authors

1 Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran

2 Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz

3 Nano biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran

4 Immunology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran

5 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz

6 Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Abstract

Background: Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM).
Objective:
To investigate the association between RM and five polymorphisms of cytokine genes, interleukin 10 (IL-10), (-592 A/C, -819 C/T, -1082 A/G), IL-6 (-174 C/G) and IL-17 (-197 G/A) in Iranian women.
Method:
Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) was performed to determine the frequencies of the IL-6, IL-10 and IL-17 gene polymorphisms in 85 women with RM compared with 104 healthy controls.
Results:
The frequencies of IL- 10 promoter gene polymorphisms (-592 A/C and -819 C/T) were significantly higher in RM women than those in controls (p=0.003). However, no statistically significant differences were observed in the frequencies of IL-6 (-174 C/G), IL-10 (-1082 A/G) and IL-17 (-197 G/A) polymorphisms between RM women and controls.
Conclusion: These results suggest that IL-10 gene polymorphism screening might have some relevance in patients with RM, a suggestion which requires further studies.

Keywords