Document Type: Original Article
Department of Immunology, Faculty of Medical Sciences, Shaheed Sadughi University of Medical Sciences, Yazd, Iran
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Masih Daneshvari Hospital, Beheshti University of Medical Sciences, Tehran, Iran
Background: Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tubercu-losis is not well known.
Objective: The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis.
Methods: In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from mul-tidrug-resistant tuberculosis (MDR-TB), TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tubercu-lin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were meas-ured by enzyme-linked immunosorbent assay.
Results: The IL-12 production by mono-cytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased (p<0.05). IL-10 production by monocytes in TB patients in response to total lipid antigens showed a significant in-crease in comparison to MDR-TB patients and healthy individuals.
Conclusion: In the MDR-TB patients, IL-10 and IL-12 production by monocytes in response to M. tubercu-losis lipid antigens are similar to the healthy subjects.