Iranian Journal of Immunology

Adverse Effect of T-2 Toxin and the Protective Role of Selenium and Vitamin E on Peripheral Blood B lymphocytes

Document Type : Short Paper

Authors

Ali Ahmadi 1
Najmeh Poursasan 2
Jafar Amani 1
Jafar Salimian 3

1 Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences

2 Institute of Biochemistry and Biophysics, University of Tehran

3 Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

https://doi.org/10.22034/iji.2015.16735
Abstract
Background: T-2 mycotoxin belongs to the Trichothecene family and has damaging effects on the immune system.
Objective: To investigate the toxic effect of T-2 toxin on the percentage of peripheral blood B lymphocytes and the potential protective role of selenium and vitamin E.
Method: Frequencies of B lymphocytes (CD19+ ) were analyzed after injection of sublethal doses of T-2 toxin into Balb/c mice at different time points, using flowcytometry. Additionally, the effects of selenium and vitamin E on B lymphocyte, as either prophylaxis or simultaneously administered with T-2 toxin, were investigated.
Results: After injection of a sublethal dose of T-2 toxin, the number of B cells (CD19+ ) significantly decreased at 12 h and became normal at 72 h. When selenium was injected both 24 h before and simultaneously with T-2 toxin, it was able to inhibit B lymphocyte (CD19+) reduction. In contrast, injecting vitamin E, 24 h before or simultaneously with T-2 toxin did not regulate B lymphocyte alteration.
Conclusion: Selenium plays pivotal role on altered B lymphocyte subset induced by T-2 toxin comparing to vitamin E.

Keywords

B Lymphocytes
Flowcytometry
Selenium
T-2 toxin
Vitamin E
Iranian Journal of Immunology
Volume 12, Issue 1
Winter 2015
Pages 64-69

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