Iranian Journal of Immunology

CTL Responses to DCs Stimulated with Leishmania Antigens Detected by DCs Expressing Leishmania gp63

Document Type : Original Article

Authors

Hossein Rezvan 1
Ali Khodadadi 2
Selman Ali 3

1 Department of Laboratory Sciences, School of Paraveterinary Sciences, Bu-Ali Sina University, Hamadan

2 Department of Immunology, School of Medicine, Jondi Shapour University, Ahwaz, Iran

3 School of Science and Technology, Nottingham Trent University, Clifton, Nottingham, UK

https://doi.org/10.22034/iji.2014.16767
Abstract
Background: Leishmania is a pathogenic parasite which infects mononuclear cells in vertebrate hosts. Different strategies have been taken to develop immunity against Leishmania . DCs loaded with immunogenic antigen have resulted in different levels of Th1-type immune response and cytotoxic T lymphocytes (CTL) activity.
Objective: To evaluate the potency of DCs primed with soluble Leishmania mexicana antigens (SLA) in developing CTL activity.
Methods: DCs were loaded with SLA and injected to Balb/c mice. After two weeks the mice were sacrificed and their splenocytes were used as effector cells in a standard 4-hour cytotoxicity assay against DCs transfected with pcDNA3 containing L. mexicana gp63 gene.
Results: Immunization of Balb/c mice with DCs loaded with SLA resulted in high levels of CTL activity against DCs transfected with pcDNA3 containing L. mexicana gp63 gene.
Conclusions: The results indicate a high potency for DCs primed with Leishmania antigens in inducing CTL activity, which can be used for developing an immunogenic vaccine against Leishmania.

Keywords

Balb/c
Cytotoxicity
DCs
gp63
Leishmania
Iranian Journal of Immunology
Volume 11, Issue 2
Spring 2014
Pages 65-73

Home

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us