Iranian Journal of Immunology

Toll Like Receptor 2 and 4 Expression in Peripheral Blood Mononuclear Cells of Multiple Sclerosis Patients

Document Type : Original Article

Authors

Seyed Javad Hasheminia 1
Sayyed Hamid Zarkesh-Esfahani 2
Sepideh Tolouei 3
Vahid Shaygannejad 4
Hedaiatallah Shirzad 5
Morteza Hashemzadeh Chaleshtory 6

1 Cellular and Molecular Research Center, School of Medicine, Shahre Kord University of Medical Sciences, Shahre Kord

2 Department of Biology, School of Sciences, University of Isfahan

3 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences

4 Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan

5 Department of Immunology, School of Medicine, Shahre Kord University of Medical Sciences

6 Cellular and Molecular Research Center, School of Medicine, Shahre Kord University of Medical Sciences, Shahre Kord , Iran

https://doi.org/10.22034/iji.2014.16768
Abstract
Background: Multiple sclerosis (MS) is a T cell mediated autoimmune disease with unknown etiology. Appropriate MS therapeutic strategies need thorough understanding of both disease etiology and pathogenesis mechanisms. Ligation of TLR-2 and TLR-4 stimulates the production of several cytokines leading to CNS autoimmunity and neurodegenerative diseases.
Objective: To find a relationship between MS disability and TLR-2 and TLR-4 expression on mononuclear cells in the blood of MS patients.
Methods: Forty-five new case (NC) MS patients (33 females and 12 males) and 45 age and gender-matched healthy controls (HC) were recruited to the study. PBMCs were prepared and the expressions of TLR-2 and TLR-4 were assessed by flowcytometry technique using appropriate monoclonal antibodies.
Results: Our results showed that the expression of TLR-2 and TLR-4 proteins in the patients group was significantly higher than that of healthy controls. TLR-2 but not TLR-4 was correlated with expanded disability status scale (EDSS) scores.
Conclusion: High expressions of TLR-2 and TLR-4 may represent a state of innate immune activation in patients with MS.

Keywords

Multiple Sclerosis
TLR-2
TLR-4
PBMC
Iranian Journal of Immunology
Volume 11, Issue 2
Spring 2014
Pages 74-83

Home

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us