Document Type : Original Article
Authors
- Seyed Javad Hasheminia 1
- Sayyed Hamid Zarkesh-Esfahani 2
- Sepideh Tolouei 3
- Vahid Shaygannejad 4
- Hedaiatallah Shirzad 5
- Morteza Hashemzadeh Chaleshtory 6
1 Cellular and Molecular Research Center, School of Medicine, Shahre Kord University of Medical Sciences, Shahre Kord
2 Department of Biology, School of Sciences, University of Isfahan
3 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences
4 Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
5 Department of Immunology, School of Medicine, Shahre Kord University of Medical Sciences
6 Cellular and Molecular Research Center, School of Medicine, Shahre Kord University of Medical Sciences, Shahre Kord , Iran
Abstract
Background: Multiple sclerosis (MS) is a T cell mediated autoimmune disease with unknown etiology. Appropriate MS therapeutic strategies need thorough understanding of both disease etiology and pathogenesis mechanisms. Ligation of TLR-2 and TLR-4 stimulates the production of several cytokines leading to CNS autoimmunity and neurodegenerative diseases.
Objective: To find a relationship between MS disability and TLR-2 and TLR-4 expression on mononuclear cells in the blood of MS patients.
Methods: Forty-five new case (NC) MS patients (33 females and 12 males) and 45 age and gender-matched healthy controls (HC) were recruited to the study. PBMCs were prepared and the expressions of TLR-2 and TLR-4 were assessed by flowcytometry technique using appropriate monoclonal antibodies.
Results: Our results showed that the expression of TLR-2 and TLR-4 proteins in the patients group was significantly higher than that of healthy controls. TLR-2 but not TLR-4 was correlated with expanded disability status scale (EDSS) scores.
Conclusion: High expressions of TLR-2 and TLR-4 may represent a state of innate immune activation in patients with MS.
Keywords
)