Iranian Journal of Immunology

IL-6, IL-10 and IL-17 Gene Polymorphisms in Iranian Women with Recurrent Miscarriage

Document Type : Original Article

Authors

Motahareh Bahadori 1
Saeed Zarei 2
Amir Hassan Zarnani 3, 4
Omid Zarei 5
Farah Idali 6
Reza Hadavi 1
Mahmood Jeddi-Tehrani 1

1 Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran

2 Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz

3 Nano biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran

4 Immunology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran

5 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz

6 Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

https://doi.org/10.22034/iji.2014.16770
Abstract
Background: Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM).
Objective: To investigate the association between RM and five polymorphisms of cytokine genes, interleukin 10 (IL-10), (-592 A/C, -819 C/T, -1082 A/G), IL-6 (-174 C/G) and IL-17 (-197 G/A) in Iranian women.
Method: Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) was performed to determine the frequencies of the IL-6, IL-10 and IL-17 gene polymorphisms in 85 women with RM compared with 104 healthy controls.
Results: The frequencies of IL- 10 promoter gene polymorphisms (-592 A/C and -819 C/T) were significantly higher in RM women than those in controls (p=0.003). However, no statistically significant differences were observed in the frequencies of IL-6 (-174 C/G), IL-10 (-1082 A/G) and IL-17 (-197 G/A) polymorphisms between RM women and controls.
Conclusion: These results suggest that IL-10 gene polymorphism screening might have some relevance in patients with RM, a suggestion which requires further studies.

Keywords

Cytokine
Gene Polymorphism
PCR-RFLP
Recurrent Miscarriage
Iranian Journal of Immunology
Volume 11, Issue 2
Spring 2014
Pages 97-104

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