Iranian Journal of Immunology

Hyperthermia Increases Natural Killer Cell Cytotoxicity against SW-872 Liposarcoma Cell Line

Document Type : Original Article

Authors

Shirin Farjadian 1
Marzie Norouzian 1
Vahid Younesi 1
Azin Ebrahimpour 1
Ramin Lotfi 2, 3

1 Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran

2 Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service BadenWürttemberg-Hessen

3 Institute of Transfusion Medicine, University of Ulm, Ulm, Germany

https://doi.org/10.22034/iji.2013.16816
Abstract
Background: Although there is convincing data in support of the effectiveness of hyperthermia in tumor therapy, the molecular mechanisms underlying the clinical effects of hyperthermia are still poorly understood.
Objective: To investigate natural killer (NK) cell cytotoxicity against heat-treated SW-872 and HeLa tumor cell lines.
Methods: NKG2D ligands and HLA class I transcription were examined using quantitative real-time PCR in treated tumor cell lines at 0, 2, 4, 6 and 12 h following thermal treatment at 39C and 42C for 1 h. The expression of MICA/B, ULBP1 and ULBP2 were also determined by flow cytometry. NK92-MI cytotoxic activity against heat-treated target cell lines was assessed by LDH release as well as annexin-V and 7-AAD assays.
Results: Our results showed that heat treatment at 39C improved the cytolytic activity of NK cells against SW-872 cells without increasing NKG2D ligand concentration or decreasing HLA class I levels.
Conclusion: The observed increase in the cytotoxicity of NK cells against SW-872 cells after hyperthermia does not coincide with changes in MICA/B, ULBP1 and ULBP2 ligands of NKG2, however, the expression of other ligands in target cells may have made the cells susceptible to the cytotoxic effect of NK cells.

Keywords

Cytotoxicity
Hyperthermia
Liposarcoma
NKG2D Ligands
SW-872
Iranian Journal of Immunology
Volume 10, Issue 2
Spring 2013
Pages 93-102

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