Iranian Journal of Immunology

MiR-143 Induces Expression of AIM2 and ASC in Jurkat Cell Line

Document Type : Original Article

Authors

Mohammad Momeni 1
Mohammad Reza Mirzaei 2
Nahid Zainodini 1
Gholamhossein Hassanshahi 2
Mohammad Kazemi Arababadi 1

1 Immunology of Infectious Diseases Research Center

2 Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

https://doi.org/10.22034/iji.2013.16817
Abstract
Background: Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play important roles in regulation of immune related genes. However, there is little information regarding the effects of miRNAs on the AIM2 and ASC expression.
Objective: To determine the mRNA levels of AIM2 and ASC in Jurkat cell line following introducing miRNA-143 (MiR-143).
Methods: MiR-143, a scrambled sequence and PBS were introduced separately, to the Jurkat cell lines and the mRNA levels of AIM2 and ASC were examined in parallel with beta-actin and GAPDH (as housekeeping genes) using Real-Time PCR technique.
Results: The mRNA levels of AIM2 and ASC were significantly increased in the MiR-143 transfected Jurkat cells when compared to the scrambled sequence or PBS treated cells.
Conclusions: MiR-143 can lead to increased expression of AIM2 and ASC mRNAs. Considering the significance of AIM2 and ASC in DNA sensing and inflammosome formation, it can be considered as a therapeutic agent for the treatment of chronic infectious diseases, especially viral infections.

Keywords

AIM2
ASC
Jurkat Cell
MiR-143
Iranian Journal of Immunology
Volume 10, Issue 2
Spring 2013
Pages 103-109

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