Document Type : Original Article
Authors
- Morteza Bagheri 1
- Ali Akbar Amirzargar 2
- Ardeshir Ghavamzadeh 3
- Kamran Alimoghadam 3
- Farideh Khosravi 2
- Bita Ansaripour 2
- Batoul Moradi 2
- Behrouz Nikbin 2
1 Department of Molecular Biology & Genetics, Uromia University of Medical Sciences, Uromia, Iran
2 Immunogenetic Laboratory, Department of Immunology, School of Medicine, Tehran University of Medical Sciences
3 Hematology-Oncology and BMT Research Center, Shariati Hospital, Tehran, Iran
Abstract
Background: β-thalassemia as a hereditary disease is defined as defective synthesis of β-globin chains, resulting in erythropoiesis abnormalities and severe anemia. Different studies have shown that cytokines and cytokine gene polymorphisms play a major role in the pathogenesis of β-thalassemia. Single nucleotide polymorphisms (SNPs) within the promoter region or other regulatory sequences of cytokine genes lead to overall production of cytokines.
Objective: To analyze the genetic profile of Th1 and Th2 cytokines in Iranian patients with β-thalassemia major.
Methods: Allelic and genotype frequencies of cytokine genes were determined in 30 thalassemia patients and 40 healthy subjects using PCR-SSP assay. Allele and genotype frequencies were calculated and compared with those of normal controls.
Results: The results of our study show a significant decrease in A allele at position UTR 5644 IFN- γ, G alleles at position -238 TNF- α and 166 IL-2, and C allele at position -590 IL-4. TGF- β haplotype TG/TG increased whereas TGF-β haplotype CG/CG and IL-10 haplotype GCC/ACC decreased significantly in all patients.
Conclusion: Data of this investigation suggest that variations among cytokine gene polymorphisms may contribute to the disease susceptibility. A finding which needs to be fairly clarified in other ethnic groups.
Keywords