Iranian Journal of Immunology

An In Vitro Assay to Evaluate the Immunomodulatory Effects of Unrestricted Somatic Stem Cells

Document Type : Original Article

Authors

Hasan Ebrahimi 1, 2
Masoud Soleimani 3
Mohammad Kazemi Arabadadi 4
Naser Ajmadbeigi 2, 3
Gholamhossein Hassanshahi 4
Akbar Farjadfar 1
Derek Kennedy 5

1 Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran

2 Stem Cell Department, Stem Cell Research Center, Tehran, Iran

3 Department of Hematology, Tarbiat Modares University, Tehran, Iran

4 Department of Microbiology, Hematology and Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

5 School of Biomolecular and Biomedical Sciences, Eskitis Institute for Cell and Molecular Therapies, Griffith University Nathan, Queensland, Australia

https://doi.org/10.22034/iji.2010.17037
Abstract
Background: Unrestricted somatic stem cells (USSC) are cord blood stem cells that have been considered as candidates for the regulation of immune responses. Therefore, potential exists for their use in the suppression of immune response after transplantation surgery.
Objective: The aim of this study was evaluation of the effect of USSC on mixed lymphocyte reaction (MLR) as a model for graft rejection.
Methods: USSC and mesanchymal stem cells (MSC) were isolated and cultured from cord blood and bone morrow, respectively. The immunophenotypes of USSC and MSC were evaluated by flow cytometery and USSC and MSC were co-cultured with peripheral blood lympho-cytes (PBL) in an MLR to evaluate the immunomodulatory effect of these cells as a percentage of the control response.
Results: Current study demonstrated that prolifera-tion of lymphocytes in the MLR was decreased after treatment with USSC, in a similar fashion to that seen with MSC.
Conclusion: It can be concluded that USSC have simi-lar regulatory effects as MSC on the MLR, which can be used as an indicator for poten-tial organ rejection after transplantation. Therefore, the immunregulatory effect of these cells could be used in the clinic during organ transplantation and in the management of autoimmunity.

Keywords

Mesanchymal Stem Cells
Mixed Lymphocyte Reaction
Transplantation
Iranian Journal of Immunology
Volume 7, Issue 1
Winter 2010
Pages 30-38

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