Iranian Journal of Immunology

Interleukin-17A and Interleukin-17F mRNA Expressions in Peripheral Blood Mononuclear Cells of Patients with Multiple Sclerosis

Document Type : Original Article

Authors

Zohreh Babaloo 1, 2
Farhad Babaie 1
Mehdi Farhoodi 2
Mohammadreza Aliparasti 1
Behzad Baradaran 1
Shohreh Almasi 1
Ahmad Hosseini 1, 3

1 Immunology Department, Medicine Faculty

2 Neuroscience Research Center, Tabriz University of Medical Sciences,Tabriz, Iran

3 Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran

https://doi.org/10.22034/iji.2010.17059
Abstract
Bakground: Multiple sclerosis (MS) is a CD4+ T cell-mediated autoimmune disease affecting the central nervous system (CNS). It was previously believed that Th1 cells were pathogenic T cells in experimental autoimmune encephalomyelitis (EAE). However, the functional role of Th1 cells in EAE has been reconsidered upon the discovery of IL-17- producing T cells which are consider as dominant effectors for inducing autoimmune tissue inflammation.
Objective: The objective of this study was to assess the role of IL-17A and IL-17F in MS pathogenesis.
Methods: We evaluated mRNA expression of IL-17A and IL-17F in thirty-five Iranian patients with relapsing–remitting MS (RRMS) and twenty-five healthy controls by Quantitative Real Time PCR.
Results: The results of this study showed a twenty-fold increase in the expression of IL-17A mRNA in MS patients compared to the control group (p < 0.0001 ). IL-17F mRNA expression in MS patients was thirty three-times greater than control group (p = 0.0008). IL-17A mRNA expression in periphery was positively correlated with expression of IL-17F transcripts in MS patients and controls (p < 0.01 and p < 0.05, respectively).
Conclusion: These results indicate the critical role of Th17- mediated cytokines in development of MS which classically has been considered as a Th1-mediated disorder. The results of this study showed, for the first time, the importance of IL-17F in MS immunopathogenesis.

Keywords

Multiple Sclerosis (MS)
IL-17A
IL-17F
mRNA gene expression
Iranian Journal of Immunology
Volume 7, Issue 4
Autumn 2010
Pages 202-209

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