Iranian Journal of Immunology

Protective Potential of Conjugated P. aeruginosa LPS –PLGA Nanoparticles in Mice as a Nanovaccine

Document Type : Original Article

Authors

Leila Safari Zanjani 1
Reza Shapoury 2
Mehrouz Dezfulian 1, 3
Mehdi Mahdavi 4
Mehdi Shafieeardestani 5

1 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran

3 Biotechnology Research Center, Karaj Branch, Islamic Azad University, Karaj, Iran

4 Pasteur Institute of Iran, Tehran, Iran

5 Department of Rhiopharmacy, Faculty of Pharmacy, Tehran of Medical Science, Tehran, Iran

https://doi.org/10.22034/iji.2020.80296
Abstract
Background: Pseudomonas aeruginosa has an important role in nosocomial infections. Objective: To evaluate biological activity of the detoxified LPS (D-LPS) entrapped into Poly lactic-co-glycolic acid (PLGA) nanoparticles. Materials: LPS was extracted and detoxified from the P. aeruginosa strain PAO1. The D-LPS, conjugated to the PLGA nanoparticles with 1-ethyl-3-dimethyl aminopropyl carbodiimide (EDAC) and N-hydroxy-succinimide (NHS). The connection was evaluated by FTIR (Fourier transform infrared), Zetasizer, and Atomic Force Microscope (AFM). The BALB/c mice injected intramuscularly with the D-LPS-PLGA with two-week intervals and then challenged two weeks after the last immunization. The bioactivity of the induced specific antisera  and cytokines responses against D-LPS-PLGA antigen was assessed by ELISA. Results: D-LPS-PLGA conjugation was confirmed by FTIR, Zetasizer, and AFM. The ELISA results showed that D-LPS was successful in the stimulation of the humoral immune response. The immune responses raised against the D-LPS-PLGA, significantly decreased bacterial titer in the spleen of the immunized mice after challenge with PAO1 strain in comparison with the control groups. Conclusion: The conjugation of the bacterial LPS to the PLGA nanoparticle increased their functional activity by decrease in bacterial dissemination and increase the killing of opsonized bacteria.

Keywords

LPS
Nanovaccine
PLGA
Pseudomonas Aeruginosa
Iranian Journal of Immunology
Volume 17, Issue 1
Winter 2020
Pages 75-86

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