Iranian Journal of Immunology

Association of rs11558471 in SLC30A8 Gene with Interleukin 17 Serum Levels and Insulin Resistance in Iranian Patients with Type 2 Diabetes

Document Type : Original Article

Authors

Mehran Ahmadi 1
Abdolkarim Mahrooz 2
Saeid Abediankenari 3
Nasim Hayati Roodbari 4

1 Department of Biochemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

3 Immunogenetics Research Center, Mazandaran University Of medical Sciences, Sari, IRAN

4 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

https://doi.org/10.22034/iji.2020.85513.1715
Abstract
Background: Functional single nucleotide polymorphisms in zinc transporter 8 (ZnT8) gene may be key determinants of humoral autoreactivity to ZnT8. Objective: The present study is expected to provide new information on the association of rs11558471 in ZnT8 gene with IL-17 levels and insulin resistance in an Iranian population [a high-risk population for type 2 diabetes (T2D)]. Methods: A total of 133 patients with T2D and 128 control subjects were included. Insulin and IL-17 concentrations were determined using ELISA. Insulin and fasting glucose levels were used to determine homeostasis model assessment for insulin resistance (HOMA-IR). The genetic analyses were performed by the restricted fragment length polymorphism (RFLP) after PCR amplification. Results: The risk allele frequency of rs11558471 in this Iranian population was among the highest in different populations. In T2D patients, compared with the GG genotypes, IL-17 concentrations were significantly higher in the GA+AA group (p= 0.042). According to the genotypes of this SNP, IL-17 concentrations, fasting glucose and HOMA-IR increased with the following order:GG

Keywords

IL-17
Insulin Resistance
rs11558471
SLC30A8
Type 2 Diabetes
Iranian Journal of Immunology
Volume 17, Issue 3
Summer 2020
Pages 215-225

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