Document Type : Original Article
Authors
- Mohammad Asgharzadeh 1
- Davoud Sanajou 2
- Hossein Samadi Kafil 3
- Mehdi Farhoudi 4
- Daryoush Savadi Oskouei 5
- Fatemeh Khaki-Khatibi 2
- Fatemeh Ahmadi 6
- Manouchehr Fadaee 7
- Ali Vegari 8
- Vahid Asgharzadeh 9
- Jalil Rashedi 10
- Behroz Mahdavi Poor 11
- Pourya Gholizadeh 12
1 Biotechnology Research Center and Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Clinical Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5 Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Department of Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
7 Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
8 Department of Medical Physics, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
9 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
10 Tabriz University of Medical Sciences
11 Department of Laboratory Sciences, School of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran
12 Tabriz University of medical sciences
Abstract
Background: Changes in the expression of cytokines as the result of the single nucleotide polymorphisms (SNPs), can affect the incidence of multiple sclerosis (MS). Objective: To investigate the relationship between the frequencies of interleukin-10 (IL-10)-1082 A/G (rs1800896) and CCR5-delta32 genotypes and susceptibility to MS in the Iranian Azari population. Methods: IL-10-1082 A/G SNP and the CCR5-delta32 were genotyped in 152 patients suffering from MS and 242 healthy non-relatives by allele specific-PCR and simple PCR methods, respectively. Results: The frequencies of AA (37.6%) and AG (55.9%) genotypes of IL-10-1082 were significantly high in the control (p = 0.021) and MS patients (p = 0.015), respectively, with no statistical difference between these groups. There was no significant difference in the CCR5 gene based on the possession of wild/wild and wild/del32 genotypes between MS patients and the control group. The del32/del32 genotype was not seen in any of the investigated groups. Tobacco (cigarettes and hookahs) consumption was higher among the MS patients (p=0.004), and this has the potential to raise the risk of MS in both the individuals and their family. However, it had no significant relation with the frequency of different genotypes of the IL-10-1082 and the CCR5. Conclusion: Our finding conclude on possible role of AA genotype of IL-10 -1082 as a protective factor in MS.
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