Iranian Journal of Immunology

Effect of the Previous History of the SARS-CoV-2 Infections on Antibody Levels among Sputnik V Vaccinated Healthcare Workers

Document Type : Original Article

Authors

Atieh Yaghoubi 1, 2
Samira Asli 3
Maryam Parhizkar 4
Maryam Mohammadpour 4
Ali Khorsand 5
Mehdi Yousefi 6
Taravat Bamdad 7
Saeid Amel Jamehdar 2

1 Antimicrobial Resistance Research Center, Mashhad University of Medical Science, Mashhad, Iran.

2 Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Clinical Research Development Center, Akbar Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

4 Hand Hygiene and Infection Control Research Center, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.

5 Department of Complementary and Chines Medicine, School of Persian and Complementary Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

6 Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

7 Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

https://doi.org/10.22034/iji.2023.93812.2260
Abstract
Background: Measuring the level of antibodies produced post-vaccination in response to the SARS-CoV-2 spike protein is considered a strategy for estimating the effectiveness of the COVID-19 vaccines.
Objective: To examine the antibody levels among the healthcare workers in different hospitals in Mashhad, Iran after receiving the second dose of Sputnik V.
Methods: In this study, we enrolled 230 healthcare workers for evaluating the Gam-COVID-Vac or Sputnik V after the second administration in different hospitals in Mashhad. Antibody levels of spike protein were quantitatively evaluated in a sample of 230 negative RT-PCR tests for the COVID-19 individuals. The analysis has been done based on an immunological assay using enzyme-linked immunosorbent assay (ELISA). The infection history of the subjects and their families was examined through their medical records.
Results: Our results demonstrated a significant association between a higher titer of IgG and a previous history of the COVID-19 infection (P<0.001). Moreover, the chance of detecting antibodies titer more than 50 AU/ml was 16.99 in these people which was significantly higher than in people without a history of infection pre-vaccination [%95CI: (7.38,39.12), P<0.001].
Conclusion: This result demonstrates that the efficacy of antibody production is related to the previous history of the SARS-CoV-2 infections. Ongoing monitoring of the level of antibody among vaccinated populations will help evaluating the effect of vaccines on humoral immunity status.

Keywords

COVID-19
Gam-COVID-Vac
SARS-CoV-2
Sputnik V
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Iranian Journal of Immunology
Volume 20, Issue 1
Winter 2023
Pages 76-82

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