Iranian Journal of Immunology

Relationship between TIM3 Expression on Peripheral T Lymphocytes and Post-Stroke Depression

Document Type : Original Article

Authors

Qifen Mao 1
Peng Zhang 2
Weicui Qi 3
Yueping Xia 3
Tingting Chen 4
Xiaofang Li 4
Songquan Xu 1
Zhiqiang Zhong 4
Zuifei Shangguan 4

1 Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, 310012, China.

2 Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, 311200, China.

3 Lishui TCM Hospital Affiliated to Zhejiang Chinese Medical University (Lishui Hospital of Traditional Chinese Medicine), Lishui, Zhejiang, 323000, China.

4 The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310006, China.

https://doi.org/10.22034/iji.2023.98917.2598
Abstract
Background: T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) is a regulatory molecule expressed on a variety of cell types, including CD3+ T cells. Few studies have been conducted to look into the correlation between TIM3 expression on peripheral T lymphocytes and post-stroke depression (PSD).
Objective: To investigate the relationship between TIM3 expressions on peripheral T lymphocytes in PSD patients.
Methods: Acute stroke patients without depression (NPSD) (n=65), PSD patients (n=23), and body mass index (BMI), age, and education-matched healthy controls (HC) (n=59) were enrolled. Using flow cytometry, TIM3 expression was examined in the peripheral CD3+ CD4+ and CD3+ CD8+ T lymphocytes. Evaluation of the depressive severity in PSD patients was assessed using a 17-item Hamilton Depression Rating Scale (HAM-D-17). We used enzyme-linked immunosorbent assay (ELISA) to determine the serum concentrations of IL-1β, IL-6, IL-10, and IL-18. We further assessed the relationships between TIM3 expression, serum cytokine levels, and the HAM-D-17 scores.
Results: CD3+ CD4+ T cells reduced significantly in PSD patients compared with the NPSD patients and HC. Both NPSD patients and PSD patients had a significant increase in TIM3 expression in their peripheral CD3+ CD4+ T lymphocytes, compared with HC. In PSD patients, a higher frequency of peripheral CD3+ CD8+ T lymphocytes showed significant expression of TIM3 compared to NPSD patients and HC. High TIM3 level on peripheral CD3+ CD8+ T lymphocytes was positively associated with the HAM-D score.
Conclusion: Patients with PSD exhibit immune dysfunction. TIM3 might contribute to the development and severity of PSD, making it a potential therapeutic target.

Keywords

Interleukin
Mucin Domain-Containing Protein 3
Post-Stroke Depression
Therapeutic Target
T Lymphocyte
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Iranian Journal of Immunology
Volume 20, Issue 4
Autumn 2023
Pages 427-437

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