Volume 17 (2020)
Volume 16 (2019)
Volume 15 (2018)
Volume 14 (2017)
Volume 13 (2016)
Volume 12 (2015)
Volume 11 (2014)
Volume 10 (2013)
Volume 9 (2012)
Volume 8 (2011)
Volume 7 (2010)
Volume 6 (2009)
Volume 5 (2008)
Volume 4 (2007)
Volume 3 (2006)
Volume 2 (2005)
Volume 1 (2004)
1. IgG Avidity Test for Ocular Toxoplasmosis Diagnosis at a Tertiary Center, Northeast of Iran

Elham Moghaddas; Seyedeh Maryam Hosseini; Karim Sharifi; Abdolrahim Rezai; Saman Soleimanpour; Mohammad Mobin Miri Moghaddam; Seyed Aliakbar Shamsian

Volume 16, Issue 3 , Summer 2019, , Pages 258-264


  Background: The diagnostic methods which are used for acute ocular toxoplasmosis are very important; if the treatment is delayed, it sometimes leads to loss of vision. Fewstudies have been performed to evaluate serological tests used in the diagnosis of acute ocular toxoplasmosis. Objective: To evaluate ...  Read More

2. A Novel mAb against a Human CD34 Peptide Reacts with the Native Protein on CD34+ Cells

Mahmood Shams; Mahmood Jeddi-Tehrani; Farzaneh Notash Haghighat; Ali Ahmad Bayat; Jafar Mahmoudian; Mohammad Reza Rezvani

Volume 10, Issue 4 , Autumn 2013, , Pages 259-266

  Background: Human CD34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (HSCs) and the small- vessel endothelial cells of a variety of tissues. CD34 plays a critical role as a marker for diagnosis and classification of leukemia. Anti CD34 antibodies are used for isolation ...  Read More

3. A Leishmania infantum FML-ELISA for the Detection of Symptomatic and Asymptomatic Canine Visceral Leishmaniasis in an Endemic Area of Iran

Behnam Mohammadi-Ghalehbin; Gholam Reza Hatam; Bahador Sarkari; Mehdi Mohebali; Zabih Zarei; Mansoureh Jaberipour; Shahab Bohlouli

Volume 8, Issue 4 , Autumn 2011, , Pages 244-250

  Background: Visceral leishmaniasis (VL) is caused by Leishmania infantum in Mediterranean basin and is an endemic disease in some parts of Iran. Canines are the main reservoirs of VL in most of the endemic areas. Different serological methods have been introduced for diagnosis of canine visceral leishmaniasis ...  Read More

4. Validation of a β-ME ELISA for Detection of Anti Leishmania donovani Antibodies in Eastern Sudan

Elfadil Abass; Abdelhafeiz Mahamoud; Durria Mansur; Mehdi Mohebali; Abdollah el Harith

Volume 8, Issue 3 , Summer 2011, , Pages 150-158

  Background: A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). Objective: The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent ...  Read More

5. Level of Interferon Gamma in the Blood of Tuberculosis Patients

Shahid Hussain; Nadeem Afzal; Khursheed Javaid; Muhammad Ikram Ullah; Tanveer Ahmad; Saleem-Uz -Zaman

Volume 7, Issue 4 , Autumn 2010, , Pages 240-246

  Background: Interferon gamma (IFN-γ), a cytokine produced by a variety of cells is involved in the immune response against M. tuberculosis. It activates the production of other cytokines and molecules that kill mycobacterium. IFN-γ also has diagnostic role in identification of active and ...  Read More

6. Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL) Levels in Peri-Implant Crevicular Fluid

Fatemeh Sarlati; Mandana Sattari; Ali Ghorbani Gazar; Ali Nabavizadeh Rafsenjani

Volume 7, Issue 4 , Autumn 2010, , Pages 226-233

  Background: Receptor activator of nuclear factor kappa B ligand (RANKL) is one of the key cytokines in the induction of osteoclastogenesis both in vitro and in vivo.Several reports indicated the presence of sRANKL in gingival crevicular fluid of patients with periodontal diseases. Objective: To determine ...  Read More

7. Comparison of Immunoblotting and ELISA for Detection of Anti-Ganglioside Antibodies in Children with Guillain-Barre Syndrome

Mohammad Reza Bonyadi; Mohammad Barzegar; Reza Badalzadeh; Mazyar Hashemilar

Volume 7, Issue 2 , Spring 2010, , Pages 117-123

  Background: Anti-ganglioside antibody assays are widely used for diagnosis of autoimmune peripheral neuropathies. Objective: This study aimed to determine serum levels of anti-ganglioside antibodies in children with Guillain-Barre syndrome by immunoblotting technique and compare the results with those ...  Read More

8. Performance of Latex Agglutination Test (KAtex) In Diagnosis of Visceral Leishmaniasis in Iran

Mohammad Amin Ghatei; Gholamreza Hatam; Seyed Mohammad Hossein Hosseini; Bahador Sarkari

Volume 6, Issue 4 , Autumn 2009, , Pages 202-207

  Background: Visceral leishmaniasis (VL) is an endemic disease in some parts of Iran. Many techniques have been used for diagnosis of VL, among which the urine based la-tex agglutination test (KAtex) is a promising one. Objective: To compare three diag-nostic tests of VL including KAtex, ELISA and Direct ...  Read More

9. Age Specific Reference Levels of Serum Prostate-Specific Antigen, Prostate Volume and Prostate Specific Antigen Density in Healthy Iranian Men

Abdol Aziz Khezri; Mehdi Shirazi; Seyyed Mohammad Taghi Ayatollahi; Mehrzad Lotfi; Mehrdad Askarian; Ali Ariafar; Mohammad Amin Afrasiabi

Volume 6, Issue 1 , Winter 2009, , Pages 40-48

  Background: It is relevant to highlight that there is not a precise and perfect report on either 95 percentile value (upper limit of normal range) or on appropriate reference intervals for serum PSA in Iranian population. Objective: To determine age-specific reference ranges for serum prostate-specific ...  Read More

10. Cloning, Expression and Purification of Truncated Chlamydia Trachomatis Outer Membrane Protein 2 (Omp2) and its Application in an ELISA Assay

Bahram Kazemi; Negar Seyed; Mojgan Bandehpour; Zarrin Sharifnia; Parviz Pakzad

Volume 5, Issue 3 , Summer 2008, , Pages 148-155

  Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the ...  Read More

11. Evaluation of Enzyme Linked Immunosorbent Assay, Utilizing Native Antigen B for Serodiagnosis of Human Hydatidosis

Seyed Mahmoud Sadjjadi; Hassan Abidi; Bahador Sarkari; Ahmad Izadpanah; Sakineh Kazemian

Volume 4, Issue 3 , Autumn 2007, , Pages 167-172

  Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not ...  Read More

12. Serum Cytokines Profiles in Iranian Patients with Preeclampsia

Reza Mansouri; Firoozeh Akbari; Mohammad Vodjgani; Fereidoun Mahboudi; Fathollah Kalantar; Mahroo Mirahmadian

Volume 4, Issue 3 , Autumn 2007, , Pages 179-185

  Background: Preeclampsia is a major cause of mortality and morbidity and is also a leading cause of preterm birth and intrauterine growth retardation. Several studies have reported abnormal levels of cytokines in women with preeclampsia. Objectives: To detect serum levels of various cytokines in pregnant ...  Read More

13. Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain

Fattaneh Mikaeili; Mahdi Fakhar; Bahador Sarkari; Mohammad H. Motazedian; Gholamreza Hatam

Volume 4, Issue 2 , Spring 2007, , Pages 116-121

  Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L. infantum) (Mediterranean type) and its major reservoir host is the dog. Ob-jective: To compare the serological methods including direct agglutination test (DAT), indirect immunofluorescent-antibody test ...  Read More

14. Immunogenicity of a Triple Diphtheria-Tetanus-Whole Cell Pertussis Vaccine in Iranian Preschool Children

Saeed Zarei; Mahmood Jeddi-Tehrani; Mohammad Mehdi Akhondi; Hojjat Zeraati; Tahere Kheirkhah; Morteza Ghazanfari; Fazel Shokri

Volume 4, Issue 2 , Spring 2007, , Pages 101-109

  Background: Immunization against diphtheria, tetanus and pertussis has been applied in Iran since 1950. WHO suggests periodical evaluation of effectiveness of the triple diphtheria-tetanus-whole cell pertussis (DTwP) vaccine, worldwide. Objectives: To determine the immunogenicity of locally manufactured ...  Read More

15. Serodiagnosis of Toxocara among Infants and Pregnant Women Suspected of Ocular or Visceral Toxocariasis Using Two Types of ELISA Antigens

Ragaa Mohamed Issa

Volume 3, Issue 4 , Autumn 2006, , Pages 176-180

  Background: The diagnosis of toxocariasis heavily depends on immunological tests because the number of parasites is usually few in infected tissues, unless they migrate into an organ such as eye. In general, patients with ocular toxocariasis have serum anti- T canis antibody titres that are significantly ...  Read More

16. Seroprevalence of Toxoplasmosis in High School Girls in Fasa District, Iran

Gholamreza Hatam; Azra Shamseddin; Farhoud Nikouee

Volume 2, Issue 3 , Summer 2005, , Pages 177-181

  Background: Toxoplasmosis is a parasitic disease caused by protozoan, Toxoplasma gondii. Infections of human are common and are usually asymptomatic. The infection may be serious if is transmitted to the fetus during pregnancy. Prophylactic measures, early detection of the infection and treatment can ...  Read More

17. Production and Characterization of Murine Monoclonal Antibodies Recognizing Conformational and Linear Epitopes Localized on Human IgA2 Molecules

Fatemeh Hajighasemi; Soheila Gharagozlou; Nasrin Moheghi; Roya Ghods; Jalal Khoshnoodi; Fazel Shokri

Volume 2, Issue 3 , Summer 2005, , Pages 125-133

  Background: There are two subclasses of human IgA (IgA1 and IgA2) that differ in antigenic properties and in chemical composition. The constant domains of α1 and α2 heavy chains have >95% sequence homology though major structural differences exist in the hinge region. Quantitation of IgA ...  Read More

18. Detection of HBV DNA in HBsAg Negative Normal Blood Donors

Abbasali Pourazar; Mansoor Salehi; Aabollah Jafarzadeh; Mohammad Kazemi Arababadi; Farzad Oreizi; Keivan Shariatinezhad

Volume 2, Issue 3 , Summer 2005, , Pages 172-176

  Background: The risk of infection by transfusion-transmitted viruses has been reduced remarkably. However, a zero-risk blood supply is still desirable. The screening for antibody to HBc (anti-HBc) has been shown as an alternative test for the detection of HBV infection. Objective: The main aim of this ...  Read More

19. Measurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method

Fatemeh Hajighasemi; Ali Akbar Saboor-Yaraghi; Fazel Shokri

Volume 1, Issue 3 , Autumn 2004, , Pages 154-161

  Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult ...  Read More

20. Assessment of Anti-streptokinase Antibody in Patients with Heart Diseases and Normal Subjects

Habibollah Saadat; Parviz Pakzad; Mandana Sattari; Negar Seyed

Volume 1, Issue 1 , Spring 2004, , Pages 63-70

  Background: Streptokinase, which is injected intravenously with a standard dose of 1.5 MIU, is the most widely used thrombolytic agent around the world. What is so important about this bioproduct is the level of antistreptokinase (anti-sk) antibody in the population, which is directly correlated to the ...  Read More

21. Detection of Rheumatoid Factors in Sera and Biopsy Lesions of Vitiligo Patients

Fatemeh Vahedi Darmian; Soheila Joubeh; Mehrnoosh Doroudchi; Behnam Abdollahi; Abbas Ghaderi

Volume 1, Issue 1 , Spring 2004, , Pages 48-55

  Background: Vitiligo is a dermatological disorder of unknown etiology with a common incidence in southern Iran. Presence of autoantibodies to melanocyte antigens suggested an autoimmune basis of the disease. Objective: In this study, the presence of rheumatoid factor (RF) in sera and skin biopsies of ...  Read More