Document Type : Original Article

Authors

• Mete Eyigor ¹
• Hulya Eyigor ²
• Ustun Osma ²
• MustafaDeniz Yilmaz ²
• Nuray Erin ³
• Omer Tarik Selcuk ²
• Cem Sezer ⁴
• Meral Gultekin ¹
• Sadi Koksoy ¹

¹ Akdeniz University Medical Faculty, Department of Medical Microbiology

² Department of ENT Head and Neck Surgery, Antalya Education and Research Hospital

³ Akdeniz University Medical Faculty, Department of Medical Pharmacology

⁴ Department of Pathology, Antalya Education and Research Hospital, Antalya, Turkey

Abstract

Background: Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation.
Objective: To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls.
Methods: Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-α (TNF-α) and Interferon-γ (IFN-γ) levels. Additionally, IL-8 levels were determined through ELISA.
Results: IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05).
Conclusion: Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.

Keywords

• Bead Array
• Cytokine
• Dysplasia
• HNSCC
• Larynxm Carcinoma